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stellaris 8 sted microscope  (Danaher Inc)


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    Danaher Inc stellaris 8 sted microscope
    Stellaris 8 Sted Microscope, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 97/100, based on 431 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stellaris+8+sted+microscope/STELLARIS+8+STED+Microscope/pm41790882-225-11-15
    Average 97 stars, based on 431 article reviews
    stellaris 8 sted microscope - by Bioz Stars, 2026-10
    97/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Standardization of cross-site biophysical studies of bovine insulin amyloids is challenged by structural polymorphism.
    Article Snippet: .. Correlative AFM-SCFM images were acquired using a NanoWizard IV AFM head (Bruker, USA) mounted on a Stellaris-8 STED microscope (Leica Microsystems, Germany). .. AFM images were collected in PBS using tapping mode with triangular silicon nitride cantilevers (NPG, Bruker, MA, USA) that have a tip curvature radius between 20 and 60 nm and a nominal spring constant of 0.24 N/m.

    Article Title: Regulation of mitochondrial DNA homeostasis by a mitochondrial microprotein
    Article Snippet: .. STimulated Emission Depletion (STED) super-resolution imaging was performed on a Leica Stellaris 8 STED microscope equipped with a tunable White Light Laser (440–790 nm) for excitation and a pulsed 775 nm STED depletion laser. ..

    Article Title: Polyarginine Peptide R11–Actin Interaction Induces a Domino Effect on Cytoskeleton Remodeling to Suppress Bladder Cancer Metastasis
    Article Snippet: Next, a 30-min incubation with TRITC–phalloidin (CA1640, Solarbio) the next day, the nuclei were stained with DAPI (40728ES03, Yeasen Biotech, Shanghai, China). .. A STELLARIS 8 STED microscope (Leica Microsystems, Germany) in STED and lightning mode with excellent lateral and axial resolution was used to collect samples in order to clearly detect the polymerization of G-actin into F-actin filaments. ..

    Article Title: Microscopy Nodes: versatile 3D microscopy visualization with Blender
    Article Snippet: .. C. Confocal and STED imaging for Fig. (Leica STELLARIS 8 STED) Microscope and optics: Leica STELLARIS 8 STED with white light laser (WLL) and pulsed STED depletion lasers at 589, 660, and 775 nm; HC PL APO CS2 86×/1.20 WATER objective; immersion medium water ( n = 1.33). ..

    Article Title: Claudin 24—A novel enhancer of AMPA receptor fidelity
    Article Snippet: Confocal image acquisition was performed on a Leica Stellaris 8 FALCON [Light Microscopy Technology Platform (LiMiTec), Core Facility for Microscopy and Imaging, Bielefeld University, Germany] using a 100×/1.4 oil objective (HC PL APO CS2 100×/1.40 OIL), with excitation at 638/582/500/405 using a white light plus diode laser. .. Stimulated emission depletion (STED) image acquisition was performed on a Leica Stellaris 8 STED microscope (Leica Microsystems CMS GmbH, Meyerhofstraße 1, 69117 Heidelberg, Germany) using a 100×/1.4 oil objective (HC PL APO CS2 100×/1.4 OIL), with excitation at 637/587/495/405 using a white light plus diode laser and STED at 775/589 nm. ..

    Article Title: Unique territorial and compartmental organization of chromosomes in the holocentric silkworm
    Article Snippet: Leica DMi8 microscope , Leica , RRID:SCR_026672. .. Leica STELLARIS 8 STED microscope , Leica , RRID:SCR_024662. ..

    Article Title: Polyarginine Peptide R11-Actin Interaction Induces a Domino Effect on Cytoskeleton Remodeling to Suppress Bladder Cancer Metastasis.
    Article Snippet: Next, a 30-min incubation with TRITC–phalloidin (CA1640, Solarbio) the next day, the nuclei were stained with DAPI (40728ES03, Yeasen Biotech, Shanghai, China). .. A STELLARIS 8 STED microscope (Leica Microsystems, Germany) in STED and lightning mode with D ow nloaded from https://spj.science.org on February 03, 2026 Liu et al. 2026 | https://doi.org/10.34133/research.1109 15 excellent lateral and axial resolution was used to collect samples in order to clearly detect the polymerization of G-actin into F-actin filaments. ..

    Imaging:

    Article Title: Regulation of mitochondrial DNA homeostasis by a mitochondrial microprotein
    Article Snippet: .. STimulated Emission Depletion (STED) super-resolution imaging was performed on a Leica Stellaris 8 STED microscope equipped with a tunable White Light Laser (440–790 nm) for excitation and a pulsed 775 nm STED depletion laser. ..

    Article Title: Microscopy Nodes: versatile 3D microscopy visualization with Blender
    Article Snippet: .. C. Confocal and STED imaging for Fig. (Leica STELLARIS 8 STED) Microscope and optics: Leica STELLARIS 8 STED with white light laser (WLL) and pulsed STED depletion lasers at 589, 660, and 775 nm; HC PL APO CS2 86×/1.20 WATER objective; immersion medium water ( n = 1.33). ..



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    (A) Three-color <t>stimulated</t> <t>emission</t> <t>depletion</t> <t>(STED)</t> imaging of shCtrl-versus shATAT1-treated iNeurons fixed on day 7 or day 14, illustrating subcellular interaction of lysosomes with ER in nanoscale. ER, acetylated and total tubulin were visualized in STED mode using antibodies against calnexin, acetyl K40 tubulin and α tubulin, respectively. Lysosomes were imaged in confocal mode and labeled with a LAMP1 antibody. (B-C) Individual fluorescence channels from (A) were segmented using CellProfiler to create object masks for quantification. Non-acetylated tubulin masks were generated by subtracting acetylated tubulin masks. Lysosome mean size was quantified from the segmented images and compared between each condition (B). The number of lysosome-ER contact sites overlapping with acetylated tubulin or non-acetylated tubulin regions were identified from segmented masks quantified in (C). Data represent mean ± SEM with one-way ANOVA (Tukey’s multiple comparison post hoc test). n (cells)=45-51 per group from three biological replicates, with data points color-coded by replicate. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001.
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    confocal stellaris 8 sted microscope  (Danaher Inc)
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    (A) Three-color <t>stimulated</t> <t>emission</t> <t>depletion</t> <t>(STED)</t> imaging of shCtrl-versus shATAT1-treated iNeurons fixed on day 7 or day 14, illustrating subcellular interaction of lysosomes with ER in nanoscale. ER, acetylated and total tubulin were visualized in STED mode using antibodies against calnexin, acetyl K40 tubulin and α tubulin, respectively. Lysosomes were imaged in confocal mode and labeled with a LAMP1 antibody. (B-C) Individual fluorescence channels from (A) were segmented using CellProfiler to create object masks for quantification. Non-acetylated tubulin masks were generated by subtracting acetylated tubulin masks. Lysosome mean size was quantified from the segmented images and compared between each condition (B). The number of lysosome-ER contact sites overlapping with acetylated tubulin or non-acetylated tubulin regions were identified from segmented masks quantified in (C). Data represent mean ± SEM with one-way ANOVA (Tukey’s multiple comparison post hoc test). n (cells)=45-51 per group from three biological replicates, with data points color-coded by replicate. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001.
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    Image Search Results


    (A) Three-color stimulated emission depletion (STED) imaging of shCtrl-versus shATAT1-treated iNeurons fixed on day 7 or day 14, illustrating subcellular interaction of lysosomes with ER in nanoscale. ER, acetylated and total tubulin were visualized in STED mode using antibodies against calnexin, acetyl K40 tubulin and α tubulin, respectively. Lysosomes were imaged in confocal mode and labeled with a LAMP1 antibody. (B-C) Individual fluorescence channels from (A) were segmented using CellProfiler to create object masks for quantification. Non-acetylated tubulin masks were generated by subtracting acetylated tubulin masks. Lysosome mean size was quantified from the segmented images and compared between each condition (B). The number of lysosome-ER contact sites overlapping with acetylated tubulin or non-acetylated tubulin regions were identified from segmented masks quantified in (C). Data represent mean ± SEM with one-way ANOVA (Tukey’s multiple comparison post hoc test). n (cells)=45-51 per group from three biological replicates, with data points color-coded by replicate. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001.

    Journal: bioRxiv

    Article Title: Tubulin acetylation governs organelle remodeling and lysosomal reformation during neuronal differentiation

    doi: 10.64898/2026.02.13.705749

    Figure Lengend Snippet: (A) Three-color stimulated emission depletion (STED) imaging of shCtrl-versus shATAT1-treated iNeurons fixed on day 7 or day 14, illustrating subcellular interaction of lysosomes with ER in nanoscale. ER, acetylated and total tubulin were visualized in STED mode using antibodies against calnexin, acetyl K40 tubulin and α tubulin, respectively. Lysosomes were imaged in confocal mode and labeled with a LAMP1 antibody. (B-C) Individual fluorescence channels from (A) were segmented using CellProfiler to create object masks for quantification. Non-acetylated tubulin masks were generated by subtracting acetylated tubulin masks. Lysosome mean size was quantified from the segmented images and compared between each condition (B). The number of lysosome-ER contact sites overlapping with acetylated tubulin or non-acetylated tubulin regions were identified from segmented masks quantified in (C). Data represent mean ± SEM with one-way ANOVA (Tukey’s multiple comparison post hoc test). n (cells)=45-51 per group from three biological replicates, with data points color-coded by replicate. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001.

    Article Snippet: Stained samples were acquired on a Leica STELLARIS 8 FALCON STED on an inverted Leica DMI8 motorized microscope (Leica microsystems) with 775nm depletion laser, White Light Lasers (WLL) for excitation, and HyD detectors using HC PL APO 100×/1.40 Oil objective.

    Techniques: Imaging, Labeling, Fluorescence, Generated, Comparison